This Article Full Text (OnlineFirst PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Stief, T. W. Search for Related Content PubMed PubMed Citation Articles by Stief, T. W. Social Bookmarking                   What's this?

The Recalcified Coagulation Activity

University Hospital

^* To whom correspondence should be addressed. E-mail: thstief{at}med.uni-marburg.de.

	Abstract

Thrombin activity generated after plasma recalcification is of analytical and clinical interest. Fifty microliters of citrated plasma was recalcified with 5 µL of 250 mM CaCl₂. After 0 to 90 minutes (37°C) 50 µl 2.5 M arginine, pH 8.6, was added. After 20 minutes, thrombin was chromogenically quantified. In normal recalcified plasma, the generated thrombin activity is about 0.1-0.2 IU/ml (37°C) when fibrin generation starts. Pooling of normal plasmas increases the generated thrombin activity about 3-fold. Plasmas of patients on heparin or coumarin generate about 10-fold less thrombin activity. Freezing of pooled plasma at -20°C and thawing at room temperature or 37°C increases thrombin generation approximately 1.5- or 2-fold, respectively. Only thrombin activities in the ascending part of the thrombin generation curve (RECA-t₂/RECAt₁>1) are valid. So a prothrombotic state in blood or plasma can be diagnosed.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?