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Homocysteine Activates Platelets In VitroAcademic Surgical Unit, Imperial College at St Mary's, London, Westmead Hospital, Wentwothville, NSW, Australia, irwin.mohan{at}doctors.org.uk
Department of Clinical Biochemistry, Royal Free Hospital, Hampstead, London, UK
Department of Clinical Biochemistry, Royal Free Hospital, Hampstead, London, UK
Academic Surgical Unit, Imperial College at St Mary's, London, University of Newcastle, Newcastle upon Tyne, UK The mechanism of thrombogenicity in hyperhomocysteinemia remains controversial. The authors investigated the association between elevated plasma homocysteine levels, platelet function, and blood coagulation. Blood was collected from healthy subjects and patients with critical limb ischemia. Basal platelet counts and platelet aggregation as well as flow cytometry were performed to assess spontaneous- and agonist-induced platelet aggregation as well as P-selectin and Glycoprotein IIb/IIIa expression at different homocysteine concentrations. Thromboelastography was performed, and platelet shape change was assessed, using a channelyzer, by measuring median platelet volume. Lactate dehydrogenase was measured, to indirectly assess red blood cell membrane integrity, after homocysteine exposure. The study results suggest that platelet activation and hypercoagulability occur after exposure to homocysteine, especially in patients with critical limb ischemia. Homocysteine concentrations of approximately 50 µmol/L appear to be the level at which these changes occur in vitro, and this effect on platelets appears to be indirect.
Key Words: homocysteine • aggregation • platelet • platelet shape change • thromboelastography
This version was published on January
1, 2008 Clinical and Applied Thrombosis/Hemostasis, Vol. 14, No. 1,
8-18 (2008) This article has been cited by other articles:
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