This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Katsura, K. Articles by Fukuhara, S. Search for Related Content PubMed Articles by Katsura, K. Articles by Fukuhara, S. Social Bookmarking                         What's this?

Platelet Procoagulant Activity During,Peripheral Blood Stem Cell Harvest

First Department of Internal Medicine, Kansai Medical University, Osaka, Japan

Shosaku Nomura

First Department of Internal Medicine, Kansai Medical University, Osaka, Japan, Department of Blood Transfusion, Kansai Medical University, Osaka, Japan

Gui Lan Xie

First Department of Internal Medicine, Kansai Medical University, Osaka, Japan

Tetsuji Ohtani

Department of Blood Transfusion, Kansai Medical University, Osaka, Japan

Tomoko Ishida

Department of Blood Transfusion, Kansai Medical University, Osaka, Japan

Hideo Kagawa

First Department of Internal Medicine, Kansai Medical University, Osaka, Japan

Chikaho Kitada

First Department of Internal Medicine, Kansai Medical University, Osaka, Japan

Yoshitaka Yamanaka

First Department of Internal Medicine, Kansai Medical University, Osaka, Japan

Hiroyuki Kitajima

First Department of Internal Medicine, Kansai Medical University, Osaka, Japan

Shirou Fukuhara

First Department of Internal Medicine, Kansai Medical University, Osaka, Japan, Department of Blood Transfusion, Kansai Medical University, Osaka, Japan

We used flow cytometry to measure platelet-derived microparticle levels in plasma obtained from 16 patients during peripheral blood stem cell harvest (PBSC) and in platelet concentrates prepared by apheresis from 10 normal controls. We also studied the binding of an anti-P-selectin antibody and annexin-V to platelets. When all 60 harvests were assessed, we noted a significant difference in microparticle levels between patients with a platelet count >10 x 10⁴/µl and those with a platelet count <10 X 10 ⁴/µl (12.3 ± 4.4 vs. 75 ± 3.9%). In both the first and total harvests, the percentage of platelets and microparticles positive for anti-P-selectin and annexin-V were significantly higher than the normal control levels. These results suggest that patients undergoing mobilization by granulocyte colony-stimulating factor (G-CSF) who have a platelet count >10 X 10 ⁴/µl are at risk of increased procoagulant activity after retransfusion following PBSC harvest. Key Words: Platelet-derived microparticle— Peripheral blood stem cell harvest—Granulocyte colony-stimulating factor.

Clinical and Applied Thrombosis/Hemostasis, Vol. 3, No. 2, 124-128 (1997)
DOI: 10.1177/107602969700300210


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?