This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (26) Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Dyszkiewicz-Korpanty, A. M. Articles by Sarode, R. Search for Related Content PubMed PubMed Citation Articles by Dyszkiewicz-Korpanty, A. M. Articles by Sarode, R. Social Bookmarking                         What's this?

Approach to the Assessment of Platelet Function: Comparison between Optical-based Platelet-rich Plasma and Impedance-based Whole Blood Platelet Aggregation Methods

Department of Medicine, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas

Eugene P. Frenkel, MD

Department of Medicine, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas

Ravindra Sarode, MD

Department of Pathology, The University of Texas Southwestern Medical Center at Dallas, Dallas, Texas

Platelet aggregation studies play an important role in the assessment of hereditary and acquired platelet function defects. The first aggregation test introduced into laboratory practice used platelet-rich plasma (PRP) where aggregation was detected by an optical method. The assessment of platelet function using whole blood (WB) aggregation by an impedance method followed up nearly 20 years later. The WB impedance aggregation assay appears to be superior to the optical method because it 1) evaluates platelets in a physiologic milieu in the presence of red and white blood cells, which are known to modulate platelet function; 2) is faster; 3) has higher sensitivity; and 4) does not require centrifugation, thus avoiding injury to platelets and loss of giant thrombocytes. These two assays were compared. Clearly, the WB impedance aggregation methodology has many advantages over the optical PRP assay for the assessment of the hyperactive platelet syndrome and the effects of anti-platelet drugs.

Key Words: Optical platelet aggregation • Whole blood impedance aggregation • Hyperactive platelet syndrome • Antiplatelet drugs

Clinical and Applied Thrombosis/Hemostasis, Vol. 11, No. 1, 25-35 (2005)
DOI: 10.1177/107602960501100103


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				G. H.R. Rao Need for a Point-of-Care Assay for Monitoring Antiplatelet and Antithrombotic Therapies Stroke, June 1, 2009; 40(6): 2271 - 2272. [Full Text] [PDF]

				L. Ang and E. Mahmud Monitoring oral antiplatelet therapy: is it justified? Therapeutic Advances in Cardiovascular Disease, December 1, 2008; 2(6): 485 - 496. [Abstract] [PDF]

				M. Lordkipanidze, C. Pharand, T. A. Nguyen, E. Schampaert, D. A. Palisaitis, and J. G. Diodati Comparison of four tests to assess inhibition of platelet function by clopidogrel in stable coronary artery disease patients Eur. Heart J., December 1, 2008; 29(23): 2877 - 2885. [Abstract] [Full Text] [PDF]

				C. Velik-Salchner, S. Maier, P. Innerhofer, W. Streif, A. Klingler, C. Kolbitsch, and D. Fries Point-of-Care Whole Blood Impedance Aggregometry Versus Classical Light Transmission Aggregometry for Detecting Aspirin and Clopidogrel: The Results of a Pilot Study Anesth. Analg., December 1, 2008; 107(6): 1798 - 1806. [Abstract] [Full Text] [PDF]

				M. Lordkipanidze, C. Pharand, E. Schampaert, J. Turgeon, D. A. Palisaitis, and J. G. Diodati A comparison of six major platelet function tests to determine the prevalence of aspirin resistance in patients with stable coronary artery disease Eur. Heart J., July 2, 2007; 28(14): 1702 - 1708. [Abstract] [Full Text] [PDF]

				B. T. Ivandic, E. Giannitsis, P. Schlick, P. Staritz, H. A. Katus, and T. Hohlfeld Determination of Aspirin Responsiveness by Use of Whole Blood Platelet Aggregometry Clin. Chem., April 1, 2007; 53(4): 614 - 619. [Abstract] [Full Text] [PDF]

				B. T. Ivandic, P. Schlick, P. Staritz, K. Kurz, H. A. Katus, and E. Giannitsis Determination of Clopidogrel Resistance by Whole Blood Platelet Aggregometry and Inhibitors of the P2Y12 Receptor Clin. Chem., March 1, 2006; 52(3): 383 - 388. [Abstract] [Full Text] [PDF]